Table 2.

The FOXC1 Binding Site and Variant Oligonucleotides

	Sequencea
Oligonucleotide	Forward	Reverse
FOXC1 site	GATCCAAAGTAAATAAACAACAGA	GATCTCTGTTGTTTATTTACTTTG
Variant:
1	GATCCAAACTAAATAAACAACAGA	GATCTCTGTTGTTTATTTAGTTTG
2	GATCCAAAGAAAATAAACAACAGA	GATCTCTGTTGTTTATTTTCTTTG
3	GATCCAAAGTTAATAAACAACAGA	GATCTCTGTTGTTTATTAACTTTG
4	GATCCAAAGTATATAAACAACAGA	GATCTCTGTTGTTTATATACTTTG
5	GATCCAAAGTAATTAAACAACAGA	GATCTCTGTTGTTTAATTACTTTG
6	GATCCAAAGTAAAAAAACAACAGA	GATCTCTGTTGTTTTTTTACTTTG
7	GATCCAAAGTAAATTAACAACAGA	GATCTCTGTTGTTAATTTACTTTG
8	GATCCAAAGTAAATATACAACAGA	GATCTCTGTTGTATATTTACTTTG
9	GATCCAAAGTAAATAATCAACAGA	GATCTCTGTTGATTATTTACTTTG

^aThe 9-bp core sequence of the in vitro–derived FOXC1 site (Pierrou et al. 1994) and of the variants is underlined; the converted base in each oligonucleotide is shown in boldface italics. Purines were converted to pyrimidines with an equivalent number of hydrogen bonds, and pyrimidines were converted to purines with an equivalent number of hydrogen bonds.