Fig. 5.

Data-driven engineering of PYR1 receptors for PFAS detection. (A) Chemical structures of selected PFAS molecules targeted for sensor design. PFNA (13), PFOS (14), PFOA (15), and PFHxS (16) are four of six PFAS molecules regulated by the US Environmental Protection Agency in drinking water. (B) PFAS-library sequence profile and binding pocket mutations in PYR1^PFAS, a pan-PFAS receptor strongly activated by any of (13–16). The library was screened against 103 unique PFAS molecules (Dataset S5). (C) Yeast-hybrid β-galactosidase colony assays show that PFNA, PFOS, PFOA, and PFHxS strongly activate PYR1^PFAS. (D) Dose-dependent response of PYR1^PFAS to four on-target PFAS molecules in cell-based β-galactosidase assays. Triplicate data points are shown. EC₅₀ values and range are shown as insets.