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. 2025 Nov 23;15:45195. doi: 10.1038/s41598-025-29411-6

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Transcriptomic analysis of MCF-7 after LBP treatment. (A) Principal component analysis (PCA) of transcriptomics between Control and LBP groups (n = 3 for each group). (B) Volcano plots of differentially expressed genes (DEGs) between Control and LBP groups. Up-regulated genes are shown in red and down-regulated genes are shown in blue. (C) Bar chart of differential genes. (D) Heatmap of DEGs between Control and LBP groups (n = 3). Gene ontology (GO) analysis of differentially up-regulated (E) and down-regulated (F) genes. In the GO analysis diagram of the whole prescription, the higher the band height is, the more significant it is. Dates were presented as the mean ± SD. *p < 0.05, ** p < 0.001.