TABLE 1.
MICs of CA(1-8)M(1-18) and PXB for A. baumannii at 107 CFU/ml under different experimental conditions
| Agenta | MIC (μM)
|
|
|---|---|---|
| CA(1-8)M(1-18) | PXB | |
| Standard conditions (Hanks+Glc) | 2 | 1 |
| NaCl (mM) | ||
| 27 | 1 | 0.25 |
| 81 | 2 | 0.5 |
| 136 | 2 | 1 |
| Divalent cations | ||
| Ca2+ (mM) | ||
| 0.05 | 2 | 1 |
| 1.0 | 8 | 2 |
| 5.0 | 16 | 4 |
| Mg2+ (mM) | ||
| 0.05 | 2 | 1 |
| 1.0 | 8 | 2 |
| 5.0 | 16 | 4 |
| EDTA (mM) | ||
| 0.25 | 1 | 0.5 |
| 1.0 | 0.5 | 0.25 |
| 5.0 | 0.25 | 0.12 |
| Polyanionsb | ||
| Heparin (mg/ml) | ||
| 0.01 | 2 | 1 |
| 0.1 | 8 | 2 |
| 1.0 | 16 | 2 |
| Alginate (mg/ml) | ||
| 0.01 | 4 | 1 |
| 0.1 | 16 | 2 |
| 1.0 | >16 | 2 |
| Uncoupler CCCP (μM)b | ||
| 12.5 | 2 | 4 |
| 50 | 4 | 4 |
| 100 | 4 | 8 |
a
Unless otherwise stated, bacteria were preincubated with the corresponding agent for 15 min prior to peptide addition.
b
Peptide and the corresponding agent were preincubated for 15 min before being added to the bacterial suspension. CCCP, carbonyl cyanide m-chlorophenylhydrazone.