FIG. 4.

Northern blot analysis. Total RNA was extracted from dsRNA-free and -containing derivatives of DK21 and several colonies obtained from anastomosis. The extracts were treated with DNase and blotted to a nylon membrane. Gels were probed with a dsRNA-specific probe (A) and with ethidium bromide (B). The arrowhead indicates the position of the dsRNA. Lanes: 1, λ/HindIII; 2, prebiotinylated DNA marker; 3, dsRNA-free derivative; 4, dsRNA-containing derivative; 5 and 6, dsRNA-free derivatives obtained from hyphal anastomosis T-DK F2 and T-DK F4, respectively; 7 to 9, dsRNA-containing derivatives obtained from hyphal anastomosis T-DK B2, T-DK C5, and T-DK D1, respectively. Numbers on the left indicate sizes in kilobases.