TABLE 1.

Characteristics of dsRNA-containing and dsRNA-free isolates of DK21

Isolatea	dsRNA	Morphologyb	Colony diam (cm)c
dsRNA-containing DK21	+	Dark red, irregular	0.86 ± 0.25d
dsRNA-free DK21	−	Pink, circular	2.34 ± 0.13
T-DK B2	+	Dark red, irregular	1.01 ± 0.33d
T-DK C5	+	Dark red, irregular	0.98 ± 0.51d
T-DK D1	+	Dark red, irregular	0.88 ± 0.08d
T-DK F1	−	Pink, circular	2.38 ± 0.04

^aF. graminearum isolates T-DK B2, T-DK C5, T-DK D1, and T-DK F1 were obtained following anastomosis between dsRNA-free T-DK and dsRNA-containing DK21.

^bdsRNA-containing isolates produced dark red pigment, and their colony shape was ameboid. dsRNA-free isolates were pink and had a circular colony shape.

^cValues are the means and standard deviations compiled from 20 independent experiments. Colony diameter was determined after 3 days of incubation on CM.

^dThe value is significantly different by Student's t test (P = 0.05) from that for the dsRNA virus-free isolate.