Representative cross-reaction analysis showing the specificity of iUPPCR for identification of serotypes of S. flexneri. Lane M, PGEM-7zf(+)/HaeIII marker; lane 1, S. flexneri 1a added to well coated with antibody against S. flexneri 1a; lane 2, S. flexneri 2a added to well coated with antibody against S. flexneri 1a; lane 3, S. flexneri 2a added to well coated with antibody against S. flexneri 2a; lane 4, S. flexneri 3a added to well coated with antibody against S. flexneri 2a; lane 5, S. flexneri 3a added to well coated with antibody against S. flexneri 3; lane 6, S. flexneri 4 added to well coated with antibody against S. flexneri 3; lane 7, S. flexneri 4 added to well coated with antibody against S. flexneri 4; lane 8, S. flexneri 5 added to well coated with antibody against S. flexneri 4; lane 9, S. flexneri 5 added to well coated with antibody against S. flexneri 5; lane 10, S. flexneri Y variant added to well coated with antibody against S. flexneri 5; lane 11, S. flexneri Y variant added to well coated with antibody against S. flexneri 6.