TABLE 1.
T-RFLP fragments and Shannon-Weaver indices obtained after HaeIII/HhaI digestion of 16S rRNA PCR products of DNA isolated from stems of E. carotovora-infected and control plants
T-RF (bp)a | Agria plantsb
|
Bionta plantsb
|
||||||
---|---|---|---|---|---|---|---|---|
Without E. carotovora
|
With E. carotovora
|
Without E. carotovora
|
With E. carotovora
|
|||||
Upper stem sections | Lower stem sections | Upper stem sections | Lower stem sections | Upper stem sections | Lower stem sections | Upper stem sections | Lower stem sections | |
39c | + | + | + | + | + | + | + | + |
41c | + | + | + | + | + | |||
43 | + | + | + | + | + | + | ||
60c | + | + | + | + | + | + | + | |
66c | + | + | + | |||||
125 | + | + | ||||||
131 | + | |||||||
134 | + | + | + | + | + | + | + | + |
137 | + | + | + | + | + | + | + | + |
141c | + | + | + | + | + | + | + | + |
144c | + | + | + | + | + | + | + | |
147 | + | + | + | + | + | + | + | + |
149 | + | |||||||
153 | + | + | ||||||
175 | + | |||||||
191c | + | + | + | |||||
200c | + | |||||||
204c | + | + | + | |||||
206 | + | + | + | + | ||||
224c | + | |||||||
294 | + | |||||||
298 | + | + | + | + | ||||
310 | + | + | + | + | + | |||
313 | + | + | + | + | + | + | ||
315 | + | + | + | |||||
318 | + | + | ||||||
335 | + | + | + | + | ||||
337 | + | + | + | + | + | + | + | + |
T-RFs derived from mitochondrial 18S rDNA (197 bp) and chloroplast 16S rDNA (303 bp) were not included in the analysis.
The Shannon-Weaver diversity indices for the treatments were calculated by determining the mean values for three replicates. The Shannon-Weaver diversity indices for Agria plants were as follows: upper stem sections without E. carotovora, 1.90; lower stem sections without E. carotovora, 1.79; upper stem sections with E. carotovora, 2.49; and lower stem sections with E. carotovora, 2.26. The Shannon-Weaver diversity indices for Bionta plants were as follows: upper stem sections without E. carotovora, 1.58; lower stem sections without E. carotovora, 1.92; upper stem sections with E. carotovora, 2.46; and lower stem sections with E. carotovora, 2.30. Within groups, the following values were not significantly different (P < 0.01): 1.90, 1.79, and 1.92; 1.79 and 1.58; and 2.49, 2.26, 2.46, and 2.30.
T-RFs for which we found corresponding isolates.