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. 2002 May;68(5):2261–2268. doi: 10.1128/AEM.68.5.2261-2268.2002

TABLE 1.

T-RFLP fragments and Shannon-Weaver indices obtained after HaeIII/HhaI digestion of 16S rRNA PCR products of DNA isolated from stems of E. carotovora-infected and control plants

T-RF (bp)a Agria plantsb
Bionta plantsb
Without E. carotovora
With E. carotovora
Without E. carotovora
With E. carotovora
Upper stem sections Lower stem sections Upper stem sections Lower stem sections Upper stem sections Lower stem sections Upper stem sections Lower stem sections
39c + + + + + + + +
41c + + + + +
43 + + + + + +
60c + + + + + + +
66c + + +
125 + +
131 +
134 + + + + + + + +
137 + + + + + + + +
141c + + + + + + + +
144c + + + + + + +
147 + + + + + + + +
149 +
153 + +
175 +
191c + + +
200c +
204c + + +
206 + + + +
224c +
294 +
298 + + + +
310 + + + + +
313 + + + + + +
315 + + +
318 + +
335 + + + +
337 + + + + + + + +
a

T-RFs derived from mitochondrial 18S rDNA (197 bp) and chloroplast 16S rDNA (303 bp) were not included in the analysis.

b

The Shannon-Weaver diversity indices for the treatments were calculated by determining the mean values for three replicates. The Shannon-Weaver diversity indices for Agria plants were as follows: upper stem sections without E. carotovora, 1.90; lower stem sections without E. carotovora, 1.79; upper stem sections with E. carotovora, 2.49; and lower stem sections with E. carotovora, 2.26. The Shannon-Weaver diversity indices for Bionta plants were as follows: upper stem sections without E. carotovora, 1.58; lower stem sections without E. carotovora, 1.92; upper stem sections with E. carotovora, 2.46; and lower stem sections with E. carotovora, 2.30. Within groups, the following values were not significantly different (P < 0.01): 1.90, 1.79, and 1.92; 1.79 and 1.58; and 2.49, 2.26, 2.46, and 2.30.

c

T-RFs for which we found corresponding isolates.