TABLE 3.
Sequence analysis of partial 16S rDNA (approximately 460 to 700 bp) of endophytic bacteria isolated from stems of potato variety Bionta
| Endophyte | Origina | Theoretical T-RFs with HaeIII/HhaI (bp) | Biocontrol activity | Sequence analysis
|
||
|---|---|---|---|---|---|---|
| Closest NCBI database match (accession no.)b | % Identity | Tentative phylogenetic group | ||||
| B1 | − | 234 | + | Unidentified bacterium (UBA011425) | 98 | Firmicutes, Bacillus-Staphylococcus group |
| B2 | − | 219 | + | Achromobacter xylosoxidans (AF302097) | 98 | beta-Proteobacteria, Alcaligenaceae |
| B3 | − | 232 | + | Bacillus macroides (MB16RNAA) | 99 | Firmicutes, Bacillus-Staphylococcus group |
| B4 | − | 229 | + | Uncultured Paenibacillus sp. (AF245034) | 89 | Firmicutes, Bacillus-Staphylococcus group |
| B5 | − | 240 | − | Uncultured Paenibacillus sp. (AF245034) | 89 | Firmicutes, Bacillus-Staphylococcus group |
| B6 | − | 244 | − | Staphylococcus warneri (SW16SRRNA) | 96 | Firmicutes, Bacillus-Staphylococcus group |
| B7 | − | 181 | − | Clavibacter michiganensis (CLBSSRRI) | 99 | Firmicutes, Actinomycetales, Microbacteriaceae |
| B8 | − | 238 | − | Staphylococcus xylosus (SS1F0916S) | 99 | Firmicutes, Bacillus-Staphylococcus group |
| B9 | − | 227 | + | Uncultured Paenibacillus sp. (AF245034) | 89 | Firmicutes, Bacillus-Staphylococcus group |
| B10 | + | 216 | + | Stenotrophomonas maltophilia (SMA293464) | 97 | gamma-Proteobacteria, Xanthomonas group |
| B12c | + | 191 | − | Agrobacterium tumefaciens (ATU16SRDF) | 99 | alpha-Proteobacteria, Rhizobiaceae |
| B13 | + | 145 | − | Microbacterium sp. (AY017057S1) | 99 | Firmicutes, Actinomycetales, Microbacteriaceae |
| B14 | + | 61 | − | Rhizobium giardinii (AF345552) | 95 | alpha-Proteobacteria, Rhizobiaceae |
| B15 | + | 39 | − | Enterobacter amnigenus (EAQ23256S1) | 100 | gamma-Proteobacteria, Enterobacteriaceae |
| B16 | + | 40 | − | Stenotrophomonas maltophilia (AF100734) | 98 | gamma-Proteobacteria, Xanthomonas group |
| B17c | + | 39 | − | Pseudomonas fluorescens (AF134705) | 99 | gamma-Proteobacteria, Pseudomonadaceae |
| B18 | + | 204 | − | Unidentified eubacterium (EUB5871) | 95 | gamma-Proteobacteria, Enterobacteriaceae |
| B19c | + | 145 | − | Flavobacterium sp. strain B17 (AB027704) | 100 | CFB group, Flavobacteriaceaed |
| B20 | + | 145 | + | Microbacterium sp. (AB004713) | 98 | Firmicutes, Actinomycetales, Microbacteriaceae |
| B21 | + | 39 | + | Stenotrophomonas maltophilia (SMA293463) | 99 | gamma-Proteobacteria, Xanthomonas group |
| B22 | + | 198 | + | Unidentified beta-proteobacterium (AB015567) | 99 | beta-Proteobacteria, Comamonadaceae |
| B23 | + | 61 | − | Ochrobactrum anthropi (OAN242576) | 100 | alpha-Proteobacteria, Rhizobiaceae |
| B24 | + | 235 | − | Uncultured bacterium IAFBD8 (AF286174) | 98 | Firmicutes, Bacillus-Staphylococcus group |
| B25c | + | 39 | − | Enterobacter cloacae (ECU65720) | 98 | gamma-Proteobacteria, Enterobacteriaceae |
a
−, isolate derived from a control plant; +, isolate derived from a plant that was infected with the pathogen.
b
NCBI, National Center for Biotechnology Information.
c
Endophyte which was found in Agria plants as well as in Bionta plants.
d
CFB, Cytophaga-Flexibacter-Bacteroides.