TABLE 1.

Relative increase in levels of protein spots 72, 90, 15, and 33 as a function of a_w

Spot	Relative abundancea at aw of 0.996, 0.95, 0.89	Apparent Mw (in thousands)b	Apparent pIb
72	2, 4, 11	40	5.5
90	1, 4, 11	36	5.0
15	2, 4, 13	70	5.5
33	4, 6, 16	57	5.5

^aThe relative abundance of each protein spot is expressed as a percentage of the total protein detected on a representative 2D PA gel by scanning densitometry as described in Materials and Methods. Cells were grown in TSB medium adjusted to an a_w value of 0.996, 0.95, or 0.89 by the addition of NaCl.

^bThe apparent M_w and pI for each protein spot were estimated by comparing mobilities in 2D gels to a variety of protein standards.