TABLE 5.

Comparison of the 16S rRNA genes from the middle layer of the TFB to the T-RFLP fingerprints from the inoculum and other parts of the reactor and sequences in the Ribosomal Database Project

Taxon and clone	Peak length (bp)a	Most similar organism	Sabb	Ic	Tc	Mc	Bc
Proteobacteria
Alpha group     TFBMC2	252	Ochrobactrum tritici strain SCII24	0.973	+d	−	+	+
Beta group
TFBMD9	208	Dechlorisoma suila strain PS	0.935	+	+	+	+
TFBMF4	165	Unidentified bacterium strain rJ13e	0.833	+	+	+	+
TFBME9	164	Ralstonia eutropha strain CH34	0.931	+	+	+	+
TFBMA4	219	Alcaligenes sp. strain NKNTAU	0.904	−	−	−	−
TFBMA5	132	Diazotroph strain BA27	0.938	−	−	−	−
Gamma group
TFBMH1	126	Enterobacter agglomerans strain AH16	0.964	−	−	+	+
TFBMF5	126	Enterobacter sp.	0.933	−	−	+	+
TFBMB11	126	Enterobacter agglomerans strain AH16	0.791	−	−	+	+
TFBMH12	126	Enterobacter agglomerans strain AH16	0.907	−	−	+	+
TFBMH2	101	Stenotrophomonas nitritireducens strain L2	0.925	−	−	−	−
TFBME10	237	Unidentified gamma proteobacterium strain R2A30	0.962	−	−	−	−
TFBME4	279	Pseudomonas sp. strain CRE 12	0.937	−	−	−	−
TFBMC1	279	Pseudomonas sp. strain CRE12	0.905	−	−	−	−
Cytophagales, TFBMF3	97	Unidentified bacterium Riz1026	0.708	−	−	−	−
Nitrospira, TFBMG11	50	Unidentified eubacterium	0.802	+	+	+	+
Planctomycetes
TFBME6	111	Gemmata obscuriglobus UQM446	0.554	−	+	−	−
TFBMI5	386	Isosphaera sp. strain Schlesner 666	0.659	−	−	−	−

^aLength of terminal restriction fragment peak.

^bSab value in the Ribosomal Database Project; a value of 0.9 or greater indicates a close match to a sequence in the database.

^cI, inoculum; T, top; M, middle; A, after air injection; B, before air injection (layers in the ICB).

^dA plus indicates that a terminal restriction fragment peak was found at this length; a minus indicates that none found.

^eSequence from activated sludge reported by other researchers.