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Sudan black B-stained preparation of root nodule contents (magnification, ×1,000). (A) Thirty-day-old colonized plant nodule bacteroids, with negligible amounts of intracellular PHB (black) accumulation noted, indicating vigorous bacteroids. (B) Thirty-day-old control plant nodule bacteroids, with greater amounts of intracellular PHB (black) accumulation noted, indicating bacteroids responding to a high C/N ratio.
