TABLE 2.

Colony blot hybridization of 32 probes derived from subtractive hybridization with a panel of E. coli strains

Straina	Serotype	Hybridizationb with colony blot pattern (fragment[s]):
		A (S-1)	B (S-2, S-10, S-30)	C (S-3, S-4, S-27, S-31)	D (S-5, S-25)	E (S-6)	F (S-7)	G (S-8)	H (S-9)	I (S-11)	J (S-12, S-13, S-14, S-15, S-32)	K (S-16)	L (S-17)	M (S-18)	N (S-19, S-20, S-21)	O (S-22)	P (S-23)	Q (S-24)	R (S-26)	S (S-28)	T (S-29)
NV32	O91:H21	+	+	+	+	+	+	+	+	+	+		+	+		+	+	+	+		+
NV74	O91:H21	+	+	+	+	+	+	+	+	+	+		+	+	+	+	+	+			+
NV127	O91:H21	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
NV197	O91:H21	+	+	+	+	+	+	+	+	+	+			+	+	+	+	+	+		+
NV200	O91:H21	+	+	+	+	+	+	+	+	+	+		+	+	+	+	+	+	+		+
CH014	O91:H21	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
CH013	O91:H10		+		+					+		+		+		+		+
CH015	OR:H16	+	+		+		+		+	+				+		+	+	+	+		+
CH016	Ox3:H−		+		+	+	+		+	+	+		+		+	+	+	+		+	+
CH017	Ont:H−		+		+	+	+	+	+	+				+		+		+
CH087	O103:H2	+	+		+		+			+	+		+	+			+	+		+
EDL933	O157:H7	+	+						+		+		+	+	+		+	+
EPEC E2348/69	O127:H6	+			+			+	+		+							+

^aThe NV strains are from animal or food origins (Table 1), and the CH strains are of hemolytic-uremic syndrome origin.

^bNo hybridization to DNA from five O6:H10 STEC strains or from strain DH5α was observed. Fragments are named S-1 to S-32. +, positive hybridization signal.