TABLE 3.

Sequence analysis of CH014-specific subtracted DNA fragments.

Group and fragment name	Sequence size (bp)	Protein with similaritya	Organism	% Nucleo- tide identity	% Amino acid identity	Locationb	EcoRI- digested fragment size(s) (kb)	HindIII-digested fragment size(s) (kb)c
I
S-1	327	Transport protein E-HlyB	EDL933(pO157)	96	98	pl	NDd	ND
S-2	305	Unknown protein	Shigella flexneri(pWR100)	92	86	pl	ND	>10
		Unknown protein L,7076	EDL933(pO157)	88	85
S-3	307	Tissue invasion protein SepA	Shigella flexneri(pWR100)		37	pO91:H21	9	8
		Serine protease EspP	EDL933(pO157)		26
S-4	160	Probable IS transposase	Shigella flexneri(pWR100)	81	57	pO91:H21	10	ND
S-5	336	Copy number control protein CopB	EPEC(pB171)	99	98	chr	>10	>10
		Copy number control protein CopB	EDL933(pO157)	93	60
S-6	281	Helicase Tral	E. coli(plasmid F)	97	97	pl	>10	10
S-7	193	Unknown protein ORF2	E. coli(plasmid IncF)	89	75	pl	9	8
S-8	287	Type I restriction enzyme EcoR124II R HsdR	E. coli(plasmid R124/3)	95	95	chr	>10	8
II
S-9	324	Probable capsid portal protein GpQ	Phage P2	94	99	pl	5	ND
S-10	327	H tail component	Phage λ	99	98	chr	>10	ND
		CP-933X tail component	EDL933	97	97
S-11	234	DNA-packaging protein	Phage λ	95	89	chr	2.5	ND
		CP-933X DNA-packaging protein	EDL933	94	89
S-12	288	CP-933X-V head-tail adapter	EDL933	98	98	chr	>10, >10	>10, >10
S-13	302	CP-933M, -N, -V, -X unknown protein	EDL933	99	97	chr	>10, >10	>10, >10
S-14	123	CP-933M, -N, -V portal protein	EDL933	95	68	chr	>10, >10	>10, >10
S-15	277	CP-933M, -N, -V unknown protein	EDL933	97	96	chr	ND	>10, >10
S-16	631	CP-933M DicA similar protein	EDL933		50	chr	<1	ND
S-17	224	CP-933N, -O, -U unknown protein	EDL933	93	90	chr	2.5	ND
S-18	295	CP-933U unknown protein	EDL933	99	98	chr	10	>10
S-19	234	CP-933C terminase	EDL933	86	94	chr	>10, >10	ND
S-20	299	CP-933C terminase	EDL933	95	97	chr	>10, >10	ND
S-21	300	CP-933C head maturation protease	EDL933	95	98	chr	>10, >10	ND
III
S-22	1,342	O island no. 154 fimbrial usher protein	EDL933	78	76	chr	5	10
		Fimbrial usher protein LpfC	Salmonella typhimurium		50
S-23	279	O island no. 134 DNA processing protein	EDL933	98	97	chr	8	ND
S-24	300	O island no. 133 unknown protein	EDL933	91	93	chr	8	ND
IV
S-25	220	General secretion pathway protein EpsK	Vibrio cholerae		36	chr	8	>10
S-26	340	Peptide synthetase McyG	“Microcystis aeruginosa”		40	chr	10, 8	ND
S-27	241					pO91:H21	7	>10
S-28	285					chr	10	ND
S-29	305					chr	10	>10, 8
S-30	ND					chr	8	8
S-31	ND					pO91:H21	10	5
S-32	ND					chr	>10, >10	>10, >10

^aDetermined by comparison of sequences and coding regions with the EMBL and GenBank DNA databases. BLAST and BLASTX network services were used. IS, insertion sequence.

^bchr, chromosome located; pl, plasmid located; pO91:H21, O91:H21-specific plasmid located.

^cTwo values mean two different-size fragments.

^dND, not determined.