FIG. 2.

Dot blot analyses of M. sexta BBMV, CHAPS-solubilized BBMV (Sol. BBMV), and purified APN with ¹²⁵I-Cry1Ac or ¹²⁵I-QNR-AAA. In most cases the amounts of proteins in rows i and ii were 5 and 1 μg, respectively; for purified APN the amounts of proteins were 2 and 1 μg in rows i and ii, respectively. Native (A) or denatured (B) proteins were spotted on PVDF membranes and damp dried under a vacuum. The membranes were incubated with ¹²⁵I-Cry1Ac (0.5 nM) or ¹²⁵I-QNR-AAA (0.5 nM) for 1 h and autoradiographed as described in Materials and Methods.