FIG. 3.

Reverse-dot blot analyses of purified toxins. Five micrograms (row i) and 1 μg (row ii) of purified toxins were dot blotted as described in the legend to Fig. 2. (A) Toxin on membrane exposed to ¹²⁵I-APN. (B) Toxin on membrane exposed to ¹²⁵I-APN plus 100 mM GalNAc. (C) Toxin on membrane exposed to denatured ¹²⁵I-APN. ¹²⁵I-APN was denatured as described in Materials and Methods. (D) Purified toxins were denatured as described in Materials and Methods before dot blotting onto the membrane and exposure to ¹²⁵I-APN. All preparations were incubated for 1 h, washed, and autoradiographed.