TABLE 1.
Summary of experiments conducted to investigate the interaction of B. thuringiensis Cry1A toxins with purified M. sexta 120-kDa APN
| Exptl method (radiolabeled protein[s]) | State of APN | State of toxin | Binding of toxin to APN | Binding of APN to Cry1Ac/binding of APN to QNR-AAA |
|---|---|---|---|---|
| Ligand blot (Cry1Ac/QNR-AAA) | Denatured | Native | Yes/Yesa | |
| Dot blot (Cry1Ac/QNR-AAA) | Native | Native | Yes/Noa | |
| Denatured | Native | Yes/Yesa | ||
| Reverse-dot blot (APN) | Native | Native | Yes/No | |
| Denatured | Yes/Yes | |||
| Reverse-dot blot (denatured APN) | Denatured | Native | Yes/Yes | |
| Reverse-ligand blot (APN) | Native | Denatured | Yes/Yes |
a
Results for Cry1Ac/results for QNR-AAA.