TABLE 2.
S. mutans whole-cell proteins up- or down-regulated following growth at pH 5.2
| Functional category | Spota | Protein identityb | RMNc | Coverage (%)d | Observed migratione
|
Theoretical migrationf
|
Peptides matchedg | MOWSE scoreh | Mean spot vol ratio, pH 5.2/7.0i | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pI | Mr | pI | Mr | ||||||||
| Cellular processes and stress proteins | 2 | DnaK protein | 00169 | 24 | 4.6 | 67.0 | 4.6 | 65.3 | 9 | 5.4 × 106 | 0.53 |
| 8 | 60-kDa chaperonin | 01340 | 26 | 4.7 | 57.6 | 4.7 | 57.1 | 8 | 3.6 × 106 | 2.53j | |
| 9 | Trigger factor, Ppiase | 01099 | 16 | 4.5 | 56.0 | 4.5 | 47.5 | 6 | 5.6 × 104 | 0.50 | |
| 11 | Cell division protein FtsZ | 01544 | 14 | 4.5 | 52.8 | 4.4 | 45.7 | 4 | 7.4 × 103 | 3.07 | |
| 14 | Cell division protein FtsA | 01545 | 14 | 5.2 | 51.5 | 5.0 | 48.7 | 4 | 9.6 × 103 | 2.35 | |
| 31 | Superoxide dismutase (Mn) | 00054 | 39 | 5.1 | 23.5 | 5.0 | 22.6 | 6 | 4.1 × 105 | 4.18j | |
| Central and intermediary metabolism | 3 | Neutral endopeptidase | 01555 | 29 | 4.7 | 65.9 | 4.7 | 71.8 | 13 | 1.8 × 1012 | 4.74j |
| 6 | Glucan-1,6-α-glucosidase | 01417 | 11 | 5.3 | 60.0 | 5.0 | 62.0 | 4 | 2.4 × 102 | 0.24 | |
| 7 | Phosphoglucomutase/phoshomannomutase | 02078 | 26 | 4.9 | 60.0 | 4.9 | 63.1 | 13 | 1.8 × 108 | 5.37j | |
| 15 | Enolase | 02125 | 19 | 4.7 | 48.6 | 4.7 | 39.5 | 6 | 6.8 × 104 | 1.70j | |
| 18 | NADP-specific glutamate dehydrogenase | 00335 | 46 | 5.8 | 47.3 | 5.5 | 46.8 | 15 | 8.4 × 1013 | 0.11j | |
| 23 | l-Lactate dehydrogenase | 00108 | 25 | 5.2 | 37.0 | 5.0 | 35.2 | 7 | 2.3 × 106 | 1.71 | |
| 24 | Enoyl-[acyl-carrier protein] reductase (NADH) | 01983 | 37 | 6.1 | 32.9 | 5.7 | 33.5 | 9 | 7.7 × 107 | 0.15j | |
| 25 | Fructose bisphosphate aldolase | 16 | 5.1 | 31.7 | 5.0 | 31.4 | 4 | 1.1 × 103 | 2.28j | ||
| 26 | Acetoin reductase | 01801 | 30 | 6.3 | 30.0 | 5.5 | 22.4 | 4 | 1.8 × 104 | 6.69j | |
| 28 | Metal dependent hydrolase | 01483 | 22 | 5.2 | 28.2 | 5.1 | 28.8 | 4 | 1.4 × 104 | 4.71 | |
| 32 | Lactoylglutathione lyase | 01881 | 33 | 5.1 | 20.5 | 4.9 | 15.3 | 5 | 4.4 × 104 | 50.63j | |
| Transport/binding proteins | 4 | Phosphoenolpyruvate: protein phosphotransferase | 00388 | 18 | 4.6 | 65.5 | 4.6 | 63.4 | 10 | 1.8 × 1011 | 0.53j |
| 17 | Multiple sugar binding transport ATP-binding transport protein MsmK | 00117 | 22 | 6.3 | 44.5 | 5.9 | 42.0 | 6 | 1.4 × 103 | 0.41 | |
| Translation | 1 | EF-G | 02018 | 28 | 4.9 | 70.5 | 4.8 | 76.7 | 15 | 2.2 × 1012 | 0.49j |
| 13 | EF-Tu | 00164 | 34 | 5.0 | 52.0 | 4.8 | 43.9 | 13 | 4.9 × 1011 | 0.66 | |
| 16 | SSU ribosomal protein S1P | 00334 | 19 | 5.1 | 48.1 | 5.0 | 43.7 | 7 | 1.6 × 105 | 0.22j | |
| 19 | EF-Ts | 01267 | 9 | 5.0 | 41.5 | 4.9 | 37.7 | 5 | 7.9 × 102 | 0.25j | |
| 21 | DNA-directed RNA polymerase alpha chain | 00821 | 27 | 4.8 | 40.1 | 4.8 | 34.6 | 7 | 6.4 × 105 | 0.03j | |
| 30 | Ribosome recycling factor | 01607 | 28 | 5.9 | 24.0 | 5.6 | 20.6 | 4 | 5.7 × 103 | 2.51 | |
| Unknown function | 5 | Hypothetical protein | 00557 | 22 | 5.8 | 65.8 | 5.7 | 67.5 | 7 | 1.8 × 107 | 4.06 |
| 27 | ORF 1749 | 01749 | 24 | 5.6 | 30.6 | 5.4 | 29.4 | 5 | 4.0 × 104 | 4.77j | |
Numbers refer to the proteins labeled in Fig. 2.
Putative functions for proteins were assigned on the basis of the presence of homologs in the database for S. mutans.
RMN, number assigned to each ORF in the database for S. mutans.
Percent amino acid coverage (amino acids in peptides observed in peptide mass fingerprint/total amino acids in translated gene sequence in the database for S. mutans).
Calculated from data shown in Fig. 2.
As given in the database for S. mutans.
Number of peptides in peptide mass fingerprint contributing to MOWSE score.
As calculated by MS-Fit software using peptide mass fingerprint data.
Ratio of IOD for each protein when extracted from S. mutans cultured at pH 5.2 or 7.0 (mean of three replicates for each growth condition).
Significantly up- or down-regulated (P < 0.05) as calculated using Student's t test.