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. 2005 Oct 31;33(19):6251–6257. doi: 10.1093/nar/gki929

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© The Author 2005. Published by Oxford University Press. All rights reserved

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(A) Schematic diagram of a synthetic Holliday Junction and the products of ATP-dependent branch migration and resolution. (B) Chromatographic scheme used to purify RecQL1. (C) Holliday junction branch migration and cleavage assay with HEK293 cell nuclear extract phosphocellulose fractions. Fractions were mixed with ³²P-labelled synthetic Holliday junctions, with added ATP as indicated. ³²P-labelled DNA products were separated by PAGE and analyzed by autoradiography.