Skip to main content
. 2005 Oct 31;33(19):6251–6257. doi: 10.1093/nar/gki929

Figure 3.

Figure 3

(A) Holliday junction branch migration assay with eluates of anti-FLAG immunoprecipitations from HEK293 cells transfected with various vectors containing FLAG-tagged proteins. Eluates were mixed with 32P-labelled synthetic Holliday junctions and ATP as indicated. 32P-labelled DNA products were separated by PAGE and analyzed by autoradiography. (B) Western blot analysis of the FLAG-tagged protein eluates used in the branch migration assay. Gel was loaded; (lane 1) FLAG-RAD54L, (lane 2) FLAG-RuvBL1, (lane 3) FLAG-RecQL1, (lane 4) FLAG-RAD51C and (lane 5) FLAG-XRCC3. Blot was probed with anti-FLAG antibody and visualized by chemiluminescence.