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. 2005 Oct 31;33(19):6251–6257. doi: 10.1093/nar/gki929

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© The Author 2005. Published by Oxford University Press. All rights reserved

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(A) RT-PCR with RNA prepared from wild type HeLa cells (lanes 1,3 and 5) or RecQL1-siRNA HeLa cells (lanes 2, 4 and 6). Primer pairs for RecQL1 were used for reactions loaded in lanes 1 and 2, RAD54L for lanes 3 and 4, and RAD51C for lanes 5 and 6. Products were separated by agarose gel electrophoresis and stained with ethidium bromide. (B) Table summarizing spontaneous and MMC induced SCE in wild type and RecQL1-siRNA HeLa cells. The mean number of SCEs per cell ± the standard deviation and the number of cells counted are shown. (C) Differentially stained chromosome spreads from wild type HeLa cells (left panel) and RecQL1-siRNA HeLa cells (right panel). This experiment was repeated 3 times with similar results.