FIG. 2.
The presence of the CceIF-5A coding sequence in genomic DNA and total RNA of C. cohnii. (Left) Genomic Southern blot of C. cohnii eIF-5A DNA. Lane 1, HindIII-digested C. cohnii genomic DNA (10 μg); lane 2, positive control, plasmid pGEM-eIF5A (100 ng). The CceIF-5A cDNA (100 ng) was used by the ECL detection system (Amersham Corporation) to produce a probe. (Right) Northern blot of C. cohnii eIF-5A mRNA. Total RNA was extracted from an asynchronous culture of C. cohnii by the LiCl method. The CceIF-5A cDNA (200 ng) was used to produce the probe by random priming with [32P]dCTP.