Figure 6.

Western blot of wild-type and mutant rDMGDH produced in E. coli. DMGDH purified from pig liver for reference (lane 1). Extracts from induced cells containing the wild-type rDMGDH vector (lane 2) and rDMGDH containing the patient mutation (lane 3) were separated by SDS-PAGE, were transferred to a polyvinyldiene fluoride membrane, and were examined using anti-DMGDH antibodies. No DMGDH-specific band was present in extracts from cells containing no plasmid or plasmid without insert (data not shown).