FIG. 2.

Time course of microaerobic hydrogenase induction in R. leguminosarum. (A) SPF25(pALPF1) cells were induced in a fermentor and amperometrically analyzed for hydrogenase activity with oxygen as the electron acceptor. Values are given as nanomoles of hydrogen per hour per milligram of protein. (B) Immunodetection of HupL in SPF25(pALPF1) cell extracts at 0, 2, 3, 4, 6, 8, 10, and 24 h of microaerobic incubation. Arrows, unprocessed (U) and processed (P) forms of HupL. Bars, standard deviations.