TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| R. leguminosarum | ||
| UPM791 | 128C53 Strr | 24 |
| SPF25 | UPM791 with PfixN::hupSL | This work |
| DG2 | UPM791 fnrN1 fnrN2 | 13 |
| E. coli | ||
| S17.1 | thi pro hsdR hsdM+recA RP4 2-Tc::Mu-Km::Tn7 | 41 |
| C2110 | polA Nalr | G. Ditta |
| Plasmids | ||
| pAL618 | Cosmid containing the UPM791 hup cluster; Tcr | 23 |
| pK18mobsac | pK18 derivative sacB; Kmr | 38 |
| pSPV4 | pMP220 derivative; Tcr | 29 |
| PCR2.1-TOPO | PCR product cloning vector; Apr | Invitrogen |
| pALPF1 | PfixN::hupSL in pAL618 | This work |
| pSPF1 | PfixN::hupSL in pSPV4 | This work |
| pKPF1 | PfixN::hupSL in pK18mobsac | This work |
| pPF1 | PfixN in PCR2.1-TOPO | This work |
| pPF2 | PfixN::hupSL in PCR2.1-TOPO | This work |
| pDH1 | hupSL in PCR2.1-TOPO | This work |
| pIM1 | ′orf3orf4 in PCR2.1-TOPO | This work |