TABLE 3.
Hydrogen metabolism in bacteroids and nodules of R. leguminosarum bv. viciae strains
| Strain | Bacteroid hydrogenase activitya
|
Nodule H2 evolutionb
|
||
|---|---|---|---|---|
| −Ni | +Ni | −Ni | +Ni | |
| UPM791 | 1,080 ± 40 | 2,930 ± 320 | 2.13 ± 0.31 | <0.25 |
| SPF25 | 2,210 ± 190 | 5,060 ± 350 | 0.34 ± 0.09 | <0.25 |
a
Values are the averages of three assays ± standard deviations and are expressed as nanomoles of hydrogen per hour per milligram of protein. +Ni, addition of 20 μM NiCl2 to the standard plant nutrient solution; −Ni, no nickel addition.
b
Data are the means of four independent determinations ± standard deviations and are expressed as micromoles of hydrogen evolved per hour per gram of nodule fresh weight.