TABLE 1.
Analysis of isolates on the basis of partial 16S rRNA gene sequences, including the closest relatives as identified by using the BLAST program in the GenBank databases
| Phylogenetic group (division, subdivision) | Isolate | Sequence length (bp) | Top match [GenBank accession no.]a | Nucleotide identity (%) |
|---|---|---|---|---|
| Acidobacteria, subdivision 1 | Ellin408 | 438 | env. WR805 [AJ292771] | 98.4 |
| Ellin457 | 429 | env. WR805 [AJ292771] | 96.7 | |
| Actinobacteria, Actinobacteridae | Ellin402 | 412 | (Dactylosporangium sp.), str. SF2809 [AB017374]b | 97.8 |
| Ellin406 | 400 | Micromonospora peucetica, str. DSM 43363 [X92603] | 93.1 | |
| Ellin410 | 400 | Micromonospora fulvoviolaceus, str. 71-m115 [X92619] | 98.4 | |
| Ellin412 | 402 | Micromonospora fulvoviolaceus, str. 71-m115 [X92619] | 99.0 | |
| Ellin422 | 403 | env. WR169 [AJ233561] | 99.5 | |
| Ellin423 | 436 | Actinoplanes sp., str. IM-3145 [AF131348] | 94.3 | |
| Ellin419 | 421 | Leifsonia poae, str. VKM Ac-1401 [AF116342] | 99.7 | |
| Ellin432 | 447 | Leifsonia poae, str. VKM Ac-1401 [AF116342] | 98.9 | |
| Ellin416 | 435 | env. WR160 [AJ233560] | 93.1 | |
| Ellin420 | 407 | env. WR160 [AJ233560] | 96.3 | |
| Ellin424 | 424 | env. WR160 [AJ233560] | 94.8 | |
| Ellin425 | 446 | env. WR160 [AJ233560] | 93.3 | |
| Ellin438 | 455 | env. WR160 [AJ233560] | 93.8 | |
| Actinobacteria, Rubrobacteridae | Ellin404 | 478 | env. S12_712 [AF078396] | 93.8 |
| Ellin442 | 433 | env. Ac18 [AF388343] | 92.2 | |
| Proteobacteria, α subclass | Ellin413 | 410 | (Methylobacterium sp.), str. 6c [AJ223453] | 99.8 |
| Ellin414 | 401 | (Methylobacterium sp.), str. 6c [AJ223453] | 98.5 | |
| Ellin426 | 400 | (Sphingomonas sp.), env. D64 [AF337832] | 96.8 | |
| Ellin431 | 426 | Afipia sp., str. G9018 [U87769] | 99.8 | |
| Ellin435 | 430 | Nitrobacter sp., str. LL [L11662] | 99.5 | |
| Ellin441 | 406 | Phenylobacterium immobile, str. E [Y18216] | 94.2 | |
| Ellin429 | 407 | env. wr0111 [AJ295550] | 98.3 | |
| Ellin452 | 414 | env. wr0111 [AJ295550] | 95.4 | |
| Proteobacteria, γ subclass | Ellin405 | 480 | env. Ac53 [AF388360] | 95.9 |
| Ellin433 | 468 | env. BIfci40 [AJ318117] | 98.7 | |
| Low-G+C-content gram-positive bacteria | Ellin411 | 432 | (Bacillus), str. G11014 [AB011787] | 98.8 |
| Ellin427 | 465 | env. C006 [AY037665] | 93.2 | |
| Verrucomicrobia, subdivision 2 | Ellin428 | 481 | env. CO19 [AF013522] | 94.1 |
a
Top match as determined by the BLAST method. The BLAST program was described by Altschul et al. (1); str. denotes 16S rRNA gene sequences from cultivated bacteria, while env. denotes sequences detected as PCR-amplified products in complex microbial communities.
b
Parentheses indicate that a genus affiliation is tentative based on 16S rRNA gene sequence similarities.