FIG. 1.

CY000-producing S. cerevisiae cells under inducing conditions. Cells were cryofixed, freeze-substituted with 0.3% uranyl acetate-0.01% glutaraldehyde in methanol, and low-temperature embedded in Lowicryl HM20. After immunogold labeling with anti-cutinase (1:250) and goat anti-rabbit with conjugated 10-nm gold (1:10), CY000 cutinase was confined in the plate-like ER. (A) Low-power magnification of a CY000-producing cell. Bar = 500 nm. (B) High-power magnification of plate-like ER (arrows) with gold particles indicating the location of CY000 cutinase. Bar = 100 nm.