FIG. 4.

Cells producing CY028 cutinase have increased levels of carbonylated proteins. (A) Amount of carbonylated proteins, as determined by binding of DNPH and measured colorimetrically by using the method of Levine et al. (17). S. cerevisiae CY028-producing cells contained about 21-fold more carbonylated protein than wild-type S. cerevisiae CY000-producing cells. (B) Corresponding immunoblot, in which bound DNPH was identified by using anti-DNPB antibodies as described by Shacter et al. (24). S. cerevisiae CY028-producing cells (lane 2) contained more carbonylated protein than wild-type S. cerevisiae CY000-producing cells (lane 1).