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. 2002 May;68(5):2428–2435. doi: 10.1128/AEM.68.5.2428-2435.2002

TABLE 2.

Rates of medium overgrowth with nontarget microorganisms in raw and pasteurized milk cultures

Type of milk No. (%) of samples contaminated under the following conditionsa:
Total no. (%) of samples affected
HEYM only
BACTEC medium only BACTEC medium + HEYM
One slope Two slopes One slope Two slopes
Raw 37 33 (28.3) 28 (11.3) 7 16 (9.3) 121 (48.9)
Pasteurized 27 13 (6.9) 152 (26.5) 17 6 (4.0) 215 (37.5)
a

For HEYM, contamination was overgrowth of slopes by fungi and/or other non-acid-fast bacteria or blue discoloration of the slopes, which is known to inhibit the growth of M. paratuberculosis. For BACTEC medium, contamination was a mixed culture (acid-fast and non-acid-fast bacteria present together) or only non-acid-fast bacteria observed upon Ziehl-Neelsen staining of growth- positive cultures. Percentages for HEYM data are averages of data for one slope and two slopes.