TABLE 3.
Transposition of the TnHad2 derivativesa
| Donor plasmid (flanking IRs) | Transposition frequencyb
|
||
|---|---|---|---|
| pBR322 | pMT1252 (Tn21) | pMT1297 (Tn1722) | |
| pMS016 (IR1 and IR3) | <3.4 × 10−8 | 1.8 × 10−2 | <8.0 × 10−7 |
| pMS015 (IR1 and IR2) | <2.5 × 10−7 | 2.0 × 10−3 | <1.7 × 10−7 |
| pMS015G (IR1 and IR2G) | <3.7 × 10−8 | 1.5 × 10−2 | <4.4 × 10−7 |
a
The donor plasmid was a pSTV29-based plasmid with an insert of the PCR-amplified 0.8-kb fragment bracketed by heterologous Tn21-like IRs (Fig. 1B). The complementing plasmid was a pBR322-based plasmid carrying the tnpR and tnpA genes of Tn21 or Tn1722.
b
The transposition frequency is expressed as the number of the Kmr transconjugants per Tpr transconjugant. All values are averages from at least three independent experiments.