Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-GFP | Clontech | #632381 |
| anti-Aox1 | This paper | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Yeast nitrogen base without amino acids or ammonium sulfate | Research Products International | Y20040 |
| Ammonium sulfate | Fisher Scientific | BP212-212 |
| 15N-labeled ammonium sulfate | Cambridge Isotope Laboratories | NLM-713-25 |
| 13C-labeled glucose | Cambridge Isotope Laboratories | CLM-1396-10 |
| 13C-labeled methanol | Cambridge Isotope Laboratories | CLM-359-5 |
| Oleic acid | Thermo Fisher | 031997.22 |
| Methanol | Fisher Chemicals | A412-4 |
| Trypsin/LysC sequencing grade protease | Promega | |
| Deposited data | ||
| Kinetic fits | This paper | https://sites.mit.edu/jhdavislab/datasets/ |
| Raw mass spectrometry data | MassiVE | ftp://massive-ftp.ucsd.edu/v07/MSV000096185/ |
| Experimental models: Organisms/strains | ||
| Komagataella phaffii: Strain: wild-type GS115 plB3::HIS4 | Subramani lab | st_JD714 |
| Komagataella phaffii: Strain: atg9Δ GS115 atg9Δ::ZeocinRplB::HIS4 | Subramani lab | st_JD715 |
| Komagataella phaffii: Strain: atg11Δ GS115 atg9Δ::ZeocinRplB::HIS4 | Subramani lab | st_JD716 |
| Komagataella phaffii: Strain: atg30Δ GS115 atg9Δ::ZeocinRplB::HIS4 | Subramani lab | st_JD719 |
| Komagataella phaffii: Strain: atg32Δ GS115 atg9Δ::ZeocinRplB::HIS4 | Subramani lab | st_JD712 |
| Komagataella phaffii: Strain: GS115 pep4-, prb1-, his4-(SMD1163) plB::HIS4 | Subramani lab | st_JD717 |
| Komagataella phaffii: Strain: inp1Δ GS115 atg9Δ::ZeocinRplB::HIS4 | Subramani lab | st_JD1038 |
| Software and algorithms | ||
| Skyline 22.2.2.501 | University of Washington; MacCoss lab | https://skyline.ms/project/home/software/Skyline/begin.view |
| Spectronaut version 15 | Biognosys | https://biognosys.com/software/spectronaut/ |
| Python version 3 | Python Software Foundation | https://www.python.org/ |
| Axios Vision 4.9.1.0 | Carl Zeiss Microscopy | Axiovision-le_se64_491_SP2.exe |
| Image Studio 6.0 | Li Cor Bio | https://www.licorbio.com/image-studio |
| Perox-per-cell v0.0.6_win_rc1 | UCSD | https://github.com/AitchisonLab/perox-per-cell |
| Other | ||
| SOLA SPE plates | Thermo Fisher | 60309-001 |
| C18 columns | Pierce | PI89873 |
| uHPLC trap column | Thermo Fisher Scientific | 164946 |
| uHPLC analytical column | Thermo Fisher Scientific | ES903 |
| Ultimate 3000 UHPLC | Thermo Scientific | |
| Orbitrap Exploris 480 | Thermo Scientific | |
| Orbitrap QE HFX | Thermo Scientific | |
| UV LED black light 150w | Elworks Black Light | |
| Axioskop 2 mot plus | Zeiss |