TABLE 3.

Methylation of 2-epi-streptamine (III) by extracts of the spectinomycin producer S. flavopersicus with Ado[¹⁴C]Met as methyl donor^a

Expt no. and reactant	Final concn (μg/ml) in reaction mixture	N-[14C]methyl product formed (cpm/10 μl)b
Expt 1
Ado[14C]Met + 2-epi-streptamine (III)	114	36,640 (51)
Expt 2
Ado[14C]Met + dl-N-methyl-2-epi-streptamine (VII + VIII)	286	19,552 (27)
Ado[14C]Met + dl-N-methyl-2-epi-streptamine	57	8,443 (12)

^aLabeled reactants and products were separated by high-voltage paper electrophoresis at pH 3.6 (15).

^bNumbers in parentheses are percentages of transfer of labeled methyl group from donor to acceptor.