Skip to main content
. 2002 May;68(5):2404–2410. doi: 10.1128/AEM.68.5.2404-2410.2002

TABLE 6.

Abilities of N-[14C]methylstreptamine analogs formed by N-methyltransferases from producers of hygromycin B and spectinomycin to serve as phosphate acceptors, catalyzed by a streptamine kinase activity in extracts of the streptomycin producer S. bikiniensisa

Expt no. and reactant Phosphorylated product (cpm/10 μl)b Rf value
Expt 1 (streptamine analogs methylated by the spectinomycin producer)
    N-[14C]methylstreptamine + MgATP 17,127 (26) 0.36
    N-[14C]methyl-2-deoxystreptamine + MgATP 0
    N-[14C]methyl-2-epi-streptamine + MgATP 0
Expt 2 (streptamine analogs methylated by the hygromycin B producer)
    N-[14C]methylstreptamine + MgATP 2,357 (7) 0.35
    N-[14C]methyl-2-deoxystreptamine + MgATP 0
    N-[14C]methyl-2-epi-streptamine + MgATP 0
a

Labeled reactants (Rf value, 0.80) and products were separated by ammoniacal phenol paper chromatography, with the indicated Rf values (15).

b

Numbers in parentheses are percentages of labeled reactant converted to phosphorylated product. For comparison the same extract converted 50% of scyllo-inosamine (IN, Fig. 2) to scyllo-inosamine-P (INP, Fig. 2).