TABLE 3.
16S rRNA sequencing results for a Diploria strigosa colony from the back reef at Water Planta
| ID% | No. of similar clones | No. of base pairs sequenced | Best-matched organism | Accession no. | Division | Location of cloneb
|
|||
|---|---|---|---|---|---|---|---|---|---|
| SW | HC | BBD | DC | ||||||
| 98 | 1 | 567 | Uncultured marine bacterium ZD0409 | AJ400350 | α-Proteobacteria | × | |||
| 96 | 1 | 568 | Uncultured marine eubacterium HstpL28 | AF159650 | α-Proteobacteria | × | |||
| 99 | 3 | 604 | Pseudoalteromonas sp. isolate PRLIST2 | Y15323 | γ-Proteobacteria | × | |||
| 99 | 2 | 610 | Pseudomonas sp. strain MBIC2027 | AB030085 | γ-Proteobacteria | × | |||
| 97 | 1 | 622 | Alteromonas macleodii strain CH-518 | Y18232 | γ-Proteobacteria | × | |||
| 99 | 1 | 423 | Alteromonas macleodii strain DSM 6062 | Y18228 | γ-Proteobacteria | × | |||
| 99 | 1 | 444 | Pseudoalteromonas sp. strain A28 | AF227238 | γ-Proteobacteria | × | |||
| 99 | 1 | 572 | Uncultured bacterium Car164 | AF285610 | γ-Proteobacteria | × | |||
| 98 | 1 | 560 | Uncultured CHAB-I-7 | AJ240911 | γ-Proteobacteria | × | |||
| 91 | 1 | 619 | Uncultured KTc0924 | AF235121 | γ-Proteobacteria | × | |||
| 97 | 1 | 511 | Uncultured marine eubacterium HstpL66 | AF159670 | γ-Proteobacteria | × | |||
| 97 | 1 | 598 | Uncultured OCS44 | AF001650 | γ-Proteobacteria | × | |||
| 99 | 1 | 611 | Unidentified bacterium | Z93992 | γ-Proteobacteria | × | |||
| 98-99 | 6 | 593 | Prochlorococcus sp. strain MIT9202 | AF115269 | Cyanobacteria | × | |||
| 99 | 3 | 531 | Prochlorococcus marinus subsp. pastoris | AF180967 | Cyanobacteria | × | |||
| 99 | 1 | 603 | Prochlorococcus sp. strain MIT9312 | AF053398 | Cyanobacteria | × | |||
| 98 | 1 | 603 | Synechococcus WH8101 | AF397728 | Cyanobacteria | × | |||
| 98-99 | 3 | 513 | Cyanophora paradoxa cyanelle | M19493 | Chloroplasts | × | × | ||
| 97 | 1 | 603 | Guillardia theta | AF041468 | Chloroplasts | × | |||
| 91 | 2 | 638 | Cyanophora paradoxa cyanelle | M19493 | Chloroplasts | × | × | ||
| 92 | 1 | 659 | Marine psychrophile SW17 | AF001368 | CFB | × | |||
| 92 | 1 | 419 | Fusibacter paucivorans | AF050099 | Firmicutes | × | |||
| 98 | 1 | 594 | Unidentified eubacterium clone SAR276 | U65915 | Planctomycetales | × | |||
| 98 | 1 | 532 | Unidentified planctomycete OM55 | U70681 | Planctomycetales | × | |||
| 96 | 1 | 548 | Uncultured eubacterium TRA2-10 | AF047642 | Unknown | × | |||
| 100 | 1 | 554 | Unidentified bacterium isolate HRV39 | Z88591 | Unknown | × | |||
| 95-96 | 3 | 347 | Uncultured marine eubacterium HstpL93 | AF159684 | γ-Proteobacteria | × | |||
| 96 | 7 | 389 | Uncultured marine eubacterium HstpL43 | AF159674 | γ-Proteobacteria | × | |||
| 93-96 | 4 | 466 | Unidentified strain NKB4 | AB013256 | γ-Proteobacteria | × | |||
| 93 | 13 | 542 | Uncultured gamma proteobacterium clone 26 | AF369718 | γ-Proteobacteria | × | |||
| 92 | 13 | 698 | Oceanospirillum linum | AF260752 | γ-Proteobacteria | × | |||
| 92 | 1 | 567 | Pseudomonas sp. strain IMT40 | AF302796 | γ-Proteobacteria | × | |||
| 92 | 1 | 471 | Pseudomonas denitrificans IAM 12023 | AB021419 | γ-Proteobacteria | × | |||
| 93 | 1 | 541 | Uncultured proteobacterium MBIC3958 | AB020600 | γ-Proteobacteria | × | |||
| 97 | 1 | 385 | Haemophilus paraphrophilus | M75042 | γ-Proteobacteria | × | |||
| 92 | 1 | 613 | Oceanospirillum maris williamsae IFO15468 | AB006763 | γ-Proteobacteria | × | |||
| 99-100 | 4 | 604 | Propionibacterium acnes | AF154832 | Firmicutes | × | |||
| 97 | 1 | 373 | Uncultured Cytophagales clone CRE-PA10 | AF141499 | CFB | × | |||
| 91 | 1 | 576 | Flexibacter aggregans | M64628 | CFB | × | |||
| 98 | 1 | 590 | Uncultured Cytophagales clone CD4E12 | AY038534 | CFB | × | |||
| 91 | 1 | 635 | Planktothrix sp. strain FPI | AF212922 | Cyanobacteria | × | |||
| 93 | 2 | 588 | Xenococcus PCC7305 | AF132783 | Cyanobacteria | × | |||
| 92 | 1 | 546 | Arctic seawater bacterium R7366 | AJ293826 | Unknown | × | |||
| 89-98 | 8 | 471 | Clostridium halophilum DSM 5387 | X77837 | Firmicutes | × | × | ||
| 88 | 1 | 370 | Eubacterium oxidoreducens strain G2-2 | AF202259 | Firmicutes | × | |||
| 96 | 1 | 605 | Uncultured Cytophaga kps30 | AF195441 | Firmicutes | × | |||
| 89-94 | 5 | 470 | Cytophaga sp. strain JTB132 | AB015260 | CFB | × | |||
| 87-93 | 3 | 535 | Cytophaga fermentans | M58766 | CFB | × | |||
| 89 | 1 | 433 | Flavobacterium columnare | AB023660 | CFB | × | |||
| 90 | 1 | 348 | Flavobacterium sp. strain A43 | AB008043 | CFB | × | |||
| 97 | 1 | 480 | Roseobacter sp. (Prionitis symbiont) | AF107210 | α-Proteobacteria | × | |||
| 91 | 1 | 596 | Unidentified proteobacterium strain BD7-3 | AB015579 | α-Proteobacteria | × | |||
| 96 | 1 | 440 | Desulfobotulus sp. strain BG14 | U85470 | δ-Proteobacteria | × | |||
| 96 | 3 | 512-607 | Uncultured epsilon proteobacterium KTc1160 | AF235116 | ɛ-Proteobacteria | × | × | ||
| 91-92 | 2 | 624 | Uncultured proteobacterium strain BD1-29 | AB015529 | ɛ-Proteobacteria | × | |||
| 94-99 | 3 | 557 | Alteromonas macleodii | AF025957 | γ-Proteobacteria | × | |||
| 94-95 | 2 | 462 | Oceanospirillum sp. strain ME113 | AJ302700 | γ-Proteobacteria | × | |||
| 92 | 2 | 499 | Neptunomonas naphthovorans | AF053734 | γ-Proteobacteria | × | |||
| 95 | 1 | 489 | Unclassified Pseudomonas group | AF102866 | γ-Proteobacteria | × | |||
| 99 | 1 | 565 | Vibrio campbelli ATCC 25920T | X74692 | γ-Proteobacteria | × | |||
| 82 | 1 | 547 | Spinacia oleracea | M21453 | Chloroplasts | × | |||
| 89 | 1 | 473 | Nephroselmis olivacea | X74754 | Chloroplasts | × | |||
| 93 | 1 | 577 | Spirochaeta litoralis | M88723 | Spirochaetales | × | |||
| 93 | 1 | 532 | Trichodesmium tenue | AF013029 | Cyanobacteria | × | |||
| 89 | 1 | 491 | Uncultured bacterium TIHP302-14 | AB031600 | Unknown | × | |||
| 94 | 2 | 525-567 | Desulfovibrio aespoeensis (isolate Aspo2) | X95230 | δ-Proteobacteria | × | × | ||
| 95 | 1 | 546 | North Sea bacterium H120 | AF069667 | Unknown | × | |||
| 88 | 1 | 626 | Unidentified bacterium strain BD2-14 | AB015542 | Unknown | × | |||
| 93 | 1 | 544 | Bacterium strain 77003 | AF227847 | Unknown | × | |||
| 90 | 1 | 356 | Marine snow bacterium Adriatic87 | AF030773 | Unknown | × | |||
| 94 | 1 | 568 | Uncultured bacterium PENDANT-24 | AF142936 | α-Proteobacteria | × | |||
| 99 | 1 | 338 | Escherichia coli K12 MG1655 | AE000345 | γ-Proteobacteria | × | |||
| 91 | 1 | 768 | Uncult. gamma proteobact. CHAB-IV-34 | AJ240917 | γ-Proteobacteria | × | |||
| 93 | 1 | 297 | Uncultured gamma proteobacterium DSS65 | AJ401386 | γ-Proteobacteria | × | |||
| 94 | 1 | 355 | Uncultured gamma proteobacterium KTc119 | AF235120 | γ-Proteobacteria | × | |||
| 94 | 4 | 612 | Chlorobium vibrioforme | Y08103 | Green Sulfur Bacteria | × | |||
| 96 | 1 | 622 | Prosthecochloris aestuarii | AJ291826 | Green Sulfur Bacteria | × | |||
| 96 | 1 | 505 | Uncultured bacillariophyte AWS98-19b | AF327029 | Chloroplasts | × | |||
| 91 | 1 | 614 | Epulopiscium sp. morphotype B | M99574 | Firmicutes | × | |||
| 97-98 | 2 | 576 | Uncultured marine eubacterium HstpL78 | AF159652 | Unknown | × | |||
a
Abbreviations are as defined in footnote a to Table 1.
b
× signs indicate whether the clone occurred in the overlying seawater (SW), healthy coral tissue (HC), black bound diseased coral tissue (BBD), or dead coral skeleton surfaces (DC).