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. 2002 Jan;70(1):218–225. doi: 10.1128/IAI.70.1.218-225.2002

FIG. 3.

FIG. 3.

LPS from P. gingivalis and C. ochracea blocked NF-κB activation in 7.7/huTLR4 cells but not in 7.7/huTLR2 cells. (A) 7.7/huTLR4 cells were exposed to either PBS or various LPS: LPS from E. coli, A. actinomycetemcomitans, and F. nucleatum (1 μg/ml) and repurified LPS from E. coli (1 μg/ml), with or without LPS from P. gingivalis or C. ochracea (10 μg/ml). (B) 7.7/huTLR2 cells were exposed to PBS, heat-killed S. aureus (1 × 108 to 2 × 108 CFU/ml), or freeze-dried P. gingivalis (10 μg/ml) with or without LPS from P. gingivalis or C. ochracea (10 μg/ml). After 18 h of incubation, the cells were stained with FITC-labeled anti-CD25 MAb and subjected to flow cytometric analysis to determine transgene expression. Activation is expressed as the fold induction of mean channel fluorescence in comparison to unstimulated cells. Representative results of one of three experiments performed are shown. E.c., E. coli; P.g., P. gingivalis; A.a., A. actinomycetemcomitans; C.o., C. ochracea; F.n., F. nucleatum; HKSA, heat-killed S. aureus.