TABLE 6.
IL-8 and MCP-1 production in the supernatant of bilayers of A549 and EAhy926 cells after infection with M. tuberculosisa
| Location of supernatant | Time (h) after infection and production (pg/mL)
|
|||
|---|---|---|---|---|
| 4
|
24
|
|||
| IL-8 | MCP-1 | IL-8 | MCP-1 | |
| Lower chamber | 763 ± 70(1) | 932 ± 47 | 1,576 ± 140 | 1,886 ± 126 |
| Upper chamber | 342 ± 31 | 471 ± 44 | 671 ± 82 | 1,063 ± 170 |
a
Cells were infected with 105 bacteria as described in Materials and Methods. Concentrations (in picograms per milliliter) before infection were as follows: IL-8, 29 ± 6 (upper chamber) and 46 ± 9 (lower chamber); MCP-1, 22 ± 6 (upper chamber) and 34 ± 4 (lower chamber). Considering transmembrane resistance and permeability to dextran, the bilayer did not leak during the course of the experiment.