FIG. 2.
RT-PCR analysis of cytokine gene expression in cultures of stimulated and nonstimulated PBMC from three T. britovi-infected donors at 1.5 months p.i. (A) and 12 months p.i. (B, C, and D) for β-actin, IL-2, IL-4, IL-5, IL-6, IL-10, and IFN-γ after 48 h of culture. Lanes 1, nonstimulated PBMC; lanes 2, PBMC stimulated with T. spiralis CWE. The lanes indicated by the arrows contained molecular weight markers.