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. 2005 Nov;17(11):2873–2885. doi: 10.1105/tpc.105.036608

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Copyright © 2005, American Society of Plant Biologists

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Figure 5. — The Accumulation of miRNAs in 35S:DCL1, P1/HC-Pro, and P1/HC-Pro × 35S:DCL1 Plants.

The accumulation of the indicated miRNAs and miRNA*s was determined from RNA gel blot analysis of low molecular weight RNA. RNA was isolated from the indicated tissues of adult plants at a late stage of flowering. The same RNA samples were fractionated on two gels. Probes to miR162, miR164, miR164*, miR167, and miR167* were hybridized to a blot derived from one gel, whereas the probe to miR172 was hybridized to a blot derived from the other gel. Ethidium bromide (EtBr) staining of the predominant RNA species in the low molecular weight fraction is shown as a loading control.