Figure 3.

Expression of the Pro_MYB98:GFP and Pro_MYB98:GUS Genes.

(A) and (B) Expression of Pro_MYB98:GFP (A) and Pro_MYB98:GUS (B) in female gametophytes at the terminal developmental stage (stage FG7). Expression is detected only in the synergid cells. The ovule in (A) is oriented like that in Figure 1A, and the ovule in (B) is oriented like that in Figure 1B. The ovule in (B) was stained in 1 mM 5-bromo-4-chloro-3-indolyl-β-glucuronic acid (X-Gluc) for 30 min.

(C) Expression of Pro_MYB98:GFP in the synergid cells in a female gametophyte at late stage FG5.

(D) Expression of Pro_MYB98:GUS in a fertilized embryo sac at 12 h after pollination. Expression is detected only in the persistent synergid cell. This ovule was stained in 1 mM X-Gluc for 4 h.

(E) Expression of Pro_MYB98:GUS in a fertilized embryo sac at 12 h after pollination. Expression is detected in the persistent synergid cell and endosperm (arrowheads). This ovule was stained in 5 mM X-Gluc for 4 h.

(F) Expression of Pro_MYB98:GFP in a fertilized embryo sac at 24 h after pollination. Expression is detected in the persistent synergid cell and endosperm (arrowheads).

(G) Expression of Pro_MYB98:GUS in the trichomes of young (<5 mm) leaves. This leaf was stained in 1 mM X-Gluc for 16 h.

(H) Opened siliques from wild-type (left) and myb98-1/+ (right) plants at 10 d after pollination. Siliques from mutant plants contain desiccated ovules (arrows).

psc, persistent synergid cell; sc, synergid cell.