Figure 10.

Extracellular ATP Attenuates FB1-Induced Cell Death in Arabidopsis Cell Cultures.

(A) Light-grown cell cultures at a density of 5% (w/v) were treated with 1 μM FB1 at time 0 and spiked with 1 mM ATP at the given times. Cells were harvested at 120 h and incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoium bromide (MTT) solution.

(B) Cell cultures were treated with 1 μM FB1 at time 0, and increasing concentrations of ATP were added 40 h later. Cells were harvested at 120 h and processed as in (A).

The formation of the purple formazan is an indication of viability; unstained cells are dead; + or – indicates addition or omission of the indicated compound, respectively.