Figure 9.

FB1 Treatment Induces the Depletion of Extracellular ATP That Precedes the Loss of Membrane Integrity.

Light-grown Arabidopsis cell cultures were treated with methanol (control) or FB1 and spiked with [γ-³²P]ATP.

(A) Growth medium aliquots from triplicate control (lanes 2 and 3) or FB1-treated (lanes 4 to 6) cultures 24 h after treatment and separated by TLC. Lane 1 contains free phosphate (PO₄) and ATP standards.

(B) Level of radioactive ATP in growth medium (as a percentage of radioactivity initially added to the cell cultures) between 16 and 24 h. Gray bars, control cultures; white bars, FB1-treated cultures.

(C) Changes in the integrity of the plasma membrane over 96 h in control (black bars) and FB1-treated (white bars) cell cultures assayed by the Evans blue method.

Data and error bars represent means ± sd (n = 3).