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. 2005 Nov;17(11):3066–3080. doi: 10.1105/tpc.105.035212

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Copyright © 2005, American Society of Plant Biologists

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Figure 9. — (At)SRC2 Is Internalized within Vacuoles.

(A) to (D) Immunolocalization in embryo cells in mature, dry Arabidopsis seeds. Sections were labeled with the combinations of antibodies indicated; SRC2 indicates anti-SRC2 recombinant protein antibody, while SRC2* indicates anti-SRC2 peptide antibody. Arrows indicate examples of PSVs within each set of images. Bars = 10 μm.

(E) and (F) Sections of mature seeds from transgenic tobacco plants expressing (At)SRC2 (E) or (At)VAP (F) were labeled with polyclonal antibodies against either (At)SRC2 and DIP (E) or (At)VAP and δ-TIP (F). Arrows, examples of colocalization of (At)SRC2 with DIP; arrowheads, colocalization of (At)VAP with δ-TIP. Bar = 10 μm for (E) and (F).

In each case, sections labeled with each antibody individually gave the same pattern as shown for that antibody in the double label images (data not shown). To the left for each set of panels is the transmitted light image, while the panel at the right represents the transmitted light plus red plus green images superimposed. Colocalization of the antibodies is indicated by yellow color.