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Quantification of ER Fluorescence for GONST1-GFP and Its Mutants.
The fluorescence of the ER relative to that of the Golgi was quantified for each of the GONST1-GFP derivatives. The relative ER fluorescence (REF) is given as a percentage of the ratio between fluorescence intensity values (arbitrary units) measured in the ER and the sum of intensity values for the ER and Golgi (see Methods). Error bars represent sd for 21 measurements.
