Table 1.
Novel Cyclotide Sequences Derived from Hybanthus Species
| Name | Hybanthus sp | 6 | 1 | 2 | 3 | 4 | 5 | 6 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I | II | III | IV | V | VI | |||||||||
| Hyfl A | floribundus E | -SIS- | C | GES | C | VYIP- | C | TVT-ALVG | C | T | C | --KDKV | C | YLN |
| Hyfl B | floribundus E | GSPIQ | C | AET | C | FIGK- | C | Y-TEEL-G | C | T | C | --TAFL | C | MKN |
| Hyfl C | floribundus E | GSPRQ | C | AET | C | FIGK- | C | Y-TEEL-G | C | T | C | --TAFL | C | MKN |
| Hyfl D | floribundus E | GSVP- | C | GES | C | VYIP- | C | F-TGIA-G | C | S | C | --KSKV | C | YYN |
| Hyfl E | floribundus E | GEIP- | C | GES | C | VYLP- | C | F----LPN | C | Y | C | --RNHV | C | YLN |
| Hyfl F | floribundus E | -SIS- | C | GET | C | TTFN- | C | WI----PN | C | K | C | NHHDKV | C | YWN |
| Hyfl G | floribundus E | XXXXX | C | AET | C | VVLP- | C | FI---VPG | C | S | C | --KSSV | C | YFN |
| Hyfl H | floribundus E | XXXXX | C | AET | C | IYIP- | C | F-TEAV-G | C | K | C | --KDKV | C | YKN |
| Hyfl I | floribundus W | G-IP- | C | GES | C | VFIP- | C | I-SGVI-G | C | S | C | --KSKV | C | YRN |
| Hyfl J | floribundus W | G-IA- | C | GES | C | AYFG- | C | WI----PG | C | S | C | --RNKV | C | YFN |
| Hyfl K | floribundus W | GT-P- | C | GES | C | VYIP- | C | F-T-AVVG | C | T | C | --KDKV | C | YLN |
| Hyfl L | floribundus W | GT-P- | C | AES | C | VYLP- | C | F-TGVI-G | C | T | C | --KDKV | C | YLN |
| Hyfl M | floribundus W | GNIP- | C | GES | C | IFFP- | C | F----NPG | C | S | C | --KDNL | C | YYN |
| Hyfl N | floribundus W | XXXXX | C | GET | C | VILP- | C | I-SAAL-G | C | S | C | --KDTV | C | YKN |
| Hyfl O | floribundus W | XXXXX | C | GET | C | VIFP- | C | I-SAAF-G | C | S | C | --KDTV | C | YKN |
| Hyfl P | floribundus W | XXXXX | C | XXX | C | VWIP- | C | I-SGIA-G | C | S | C | --KNKV | C | YLN |
| Hymo A | monopetalus | XXXXX | C | GET | C | LFIP- | C | IFS--VVG | C | S | C | --SSKV | C | YRN |
| Hymo B | monopetalus | XXXXX | C | GET | C | VTGT- | C | Y-T---PG | C | A | C | --DWPV | C | KRD |
| Hyst A | stellarioides | XXXXX | C | GET | C | IWGR- | C | Y-SENI-G | C | H | C | --GFGI | C | TLN |
| Hyve A | vernonii | XXXXX | C | GET | C | LFIP- | C | LTS--VFG | C | S | C | --KNRG | C | YKI |
| Hyca A | calycinus | XXXXX | C | GET | C | VVDTR | C | Y-T---KK | C | S | C | --AWPV | C | MRN |
| Hyde A | debilissimus | XXXXX | X | XXX | C | VWIP- | C | I-S-AAIG | C | S | C | --KSKV | C | YRN |
| Hyen A | enneaspermus | XXXXX | C | GES | C | VYIP- | C | TVT-ALLG | C | S | C | --KDKV | C | YKN |
| Hyen B | enneaspermus | XXXXX | C | GET | C | KVTKR | C | --SGQ--G | C | S | C | -LKGRS | C | Y-D |
| Hyep A | epacroides | XXXXX | C | GET | C | VVLP- | C | FI---VPG | C | S | C | --KSSV | C | YFN |
| Hyep B | epacroides | XXXXX | C | GET | C | IYIP- | C | F-TEAV-G | C | K | C | --KDKV | C | YKN |
Sequences are aligned based on the conserved Cys residues (I to VI), and loops are numbered as in Figure 1. Hyfl A-C were identified by peptide sequencing. An oxidized Met in Hyfl B is shown underlined to indicate that only the oxidized form was detected. By contrast, both oxidized and reduced forms were detected for Hyfl C. The remaining sequences were identified by PCR as full or partial mRNA transcripts. XXXXX represents an undetermined section of sequence, outside that detected by the primer.