Fig. 3.

Regional distribution of protease-resistant PrP^Sc in the brain of tg338 mice infected with discordant (A and B) and Nor98 (C and D) agent. Histoblots of coronal sections were immunostained for PrP^res as described in Materials and Methods. Sections through the thalamus/hippocampus (A and C) and the pons/cerebellum (B and D) are presented. Note the concordant patterns of PrP^res accumulation for the two types of agent, with the preferential involvement of the thalamus (th), corpus callosum (cc), cerebral cortex (arrow), and, to a lesser extent, of the superior colliculus (sc; B-D). Histoblots performed on the brain of a tg338 mice inoculated with a conventional scrapie isolate (PG127) are shown for comparison (E and F).