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. 2005 Oct 18;102(44):15971–15976. doi: 10.1073/pnas.0503868102

Fig. 4.

Fig. 4.

IWe and IWeT steps in megacloning. I-PpoI fragments from indicated BGM strains run on CHEF gels with size markers indicated to the right. (Upper Left) I-PpoI fragments of cloned region [I] indicated by green arrowheads. (Upper Right) Strains of IWeT-mediated integration and elongation of region [IV] in BEST7324 (BEST7155 plus sll1652 disrupted by bsr) to BEST7374. (Lower Left) Strains of IWeT-mediated integration and elongation of region [II] in BEST7527 to BEST7566. (Lower Right) IWeT-mediated integration and elongation of region [III]. Indicated strains of IWeT of region [III] are separated into preintegration (lanes 1-4) and postintegration (lanes 5-9) of region [II]. Synechocystis genome DNA and the BGM vector part are indicated by differently colored arrowheads. Six I-PpoI fragments produced from BEST7613 are mapped to those in Fig. 2. Running conditions are shown. Yeast chromosomal DNA was run as a size marker.