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. 2002 Jan;70(1):153–162. doi: 10.1128/IAI.70.1.153-162.2002

FIG. 1.

FIG. 1.

Challenge of immune mice with actA-deficient listeriae and that with WT listeriae generate similar numbers of antigen-specific CD8+ T cells. BALB/c mice were immunized with 106 CFU of actA-deficient L. monocytogenes and challenged 28 days later with either 1.5 × 105 CFU of L. monocytogenes NPs or 6 × 106 CFU of actA-deficient L. monocytogenes NPs. The number of LLO91-99-specific (A and B) or NP118-126-specific (A and C) CD8+ T cells per spleen was determined 7 days after challenge by intracellular cytokine staining for IFN-γ. (A) Data are from a representative mouse challenged with actA-deficient L. monocytogenes NPs and stimulated with no peptide (top), LLO91-99 (middle), or NP118-126 (bottom). The number within the gate is the percentage of CD8+ T cells producing IFN-γ. Data are from one of three independent experiments with three mice per group. ns, no significant difference between values.