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. 2002 Jan;70(1):153–162. doi: 10.1128/IAI.70.1.153-162.2002

FIG. 6.

FIG. 6.

Mice immunized with 500 CFU of actA-deficient L. monocytogenes generate more CD8+ T cells specific for an antigen introduced during challenge than mice immunized with 106 CFU of actA-deficient L. monocytogenes. BALB/c mice were immunized with either 500 or 106 CFU of actA-deficient L. monocytogenes. The mice were challenged 28 days later with 2 × 105 CFU of L. monocytogenes NPns (A) or 1 × 105 CFU of L. monocytogenes NPs (B). Naïve BALB/c mice were immunized with 2 × 103 CFU of L. monocytogenes NPns (A) or 1 × 103 CFU of L. monocytogenes NPs (B). The number of NP118-126-specific CD8+ T cells per spleen was determined by intracellular cytokine staining for IFN-γ 7 days after challenge. Data are from a representative experiment of three (A) or two (B) independent experiments and are means ± SDs for three mice/group.