FIG. 4.

Scatter plots of BrdU and CD62L staining. Spleen cells were stained with monoclonal antibodies specific for CD4, CD62L, and BrdU. CD4⁺ cells were gated, and the results of staining for CD62L (Mel-14) (y axis) and BrdU (x axis) are shown. The percentage of cells falling in each quadrant is shown for each time point after infection. Similar results were obtained when CD8⁺ T cells were stained and gated in the same manner. (A) Negative control; (B) uninfected; (C) 7 weeks after infection; (D) 21 weeks after infection; (E) 36 weeks after infection. FITC, fluorescein isothiocyanate.